Using Cellprofiler to count viral particles (IV)

Identifying the Particles In the first three episodes of this tutorial, the groundwork has been laid to carry out the real task at hand, namely to identify the spots in this image as viral particles and then to count them. In this post, I’ll describe the process I went through to configure each of the many Read more about Using Cellprofiler to count viral particles (IV)[…]

Using Cellprofiler to count viral particles (III)

DETAILS-ANALYSIS MODULES This is the third post in this series of how to count viral particles with Cellprofiler. With the “ColorToGray” analysis module selected, the options in the image to the left are available for me to use to properly configure this module. I first select “StartingImage” from the popup menu that allows me to Read more about Using Cellprofiler to count viral particles (III)[…]

Using Cellprofiler to count viral particles (II)

DETAILS-INPUT MODULES This is the second post in a series that will eventually describe the entire process of counting viral particles. Before we can start analysing images, we have to get the image data into Cellprofiler. The program uses a series of input modules to do this. After correctly configuring the “Input modules” section of Cellprofiler Read more about Using Cellprofiler to count viral particles (II)[…]

TDE Mounting

Excise intestine in 0.5cm sized pieces. Fix by immersion in 4% Paraformaldehyde in PBS, overnight at 4°C, slowly rocking. I would do this so that each piece of intestine is in a 2ml eppendorf tube. Slice tissue into 2mm X 2mm pieces. Rinse 3X 20min per rinse in PBS, to remove PFA Permeabilise in 1% Read more about TDE Mounting[…]