Sample Preparation for STED Microscopy

Please download the following pdf file and read it before attempting any immunofluorescence protocols to be used on the STED microscope. Leica will be shipping some of the specially required fluorochromes conjugated to ant-mouse and anti-rabbit antibodies, so if you have a requirement for an antibody against an unusual primary species please let me know ass soon as possible.

2-color STED CW sample preparation V12 October 2011